Protocols

DNA/RNA extraction

DNA/RNA co-extraction (pine roots)

DNA/RNA extraction

1-step PCR approach: https://cbs.umn.edu/kennedy-lab/protocols

2-step PCR approach: 

3-step PCR approach: See supplementary text on: Chen K-H, Liao H-L, Arnold AE, Bonito G, Lutzoni F. 2018. RNA-based analyses reveal fungal communities structured by a senescence gradient in the moss Dicranum scoparium and the presence of putative multi-trophic fungi. New Phytologist 218: 1597–1611doi: 10.1111/nph.15092

Metatranscriptomic analysis

The tools applied for metatranscriptomic analysis please see: https://sunnymicrobiome.weebly.com/