SOIL AND WATER SCIENCES DIAGNOSTIC LABORATORIES
Faculty and students in the Department of Soil, Water, and Ecosystem Sciences use a wide range of analytical and field instrumentation in their research programs. The department established several core laboratories, not only to support faculty and students but also to provide analytical services and expertise to our clientele in the State of Florida and elsewhere. This group of Soil and Water Diagnostic Laboratories houses state-of-the-art instrumentation for the analysis of soil, water, plant, and other environmental samples for plant nutrients and a range of inorganic and organic contaminants. These services are available at a nominal cost to our clientele.
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Mineralogy and Soil Chemistry Laboratory
The Pedology and Mineralogy Core Laboratory provides basic physical, chemical, and mineralogical analyses for soils and sediments, performing the suite of analyses generally included under the term “soil characterization,” some of which are needed to classify soils under USDA-NRCS soil taxonomy. The laboratory houses a variety of instruments, including a computer-controlled x-ray diffractometer, petrographic and dissecting microscopes (with digital camera), UV-visible spectrophotometers, and other basic laboratory equipment.
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GIS and Spectral Soil Laboratory
The GIS and Spectral Soil Laboratory provides state-of-the art setup for landscape modeling, soil and remote sensing, and environmental data analysis (statistical analyses; geostatistical modeling; space-time modeling; geographic information systems (GIS); remote sensing; and mechanistic simulation modeling). Modern quantitative methods are used to support digital soil mapping and modeling projects. The spectral soil lab houses a spectroradimeter (QualitySpec Pro; Analytical Spectral Devices, Inc.) for hyperspectral visible/near-infrared diffuse reflectance spectroscopic measurements and a FTIR (with drift accessory) 600 series (Agilent Technologies Inc.) for hyperspectral mid-infrared sensing.
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ICP - Mass Spectrometry Laboratory
The Biogeochemistry of Trace Metals Laboratory (BTML) provides expertise and service for elemental analysis of environmental samples.
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Microbial Ecology and Molecular Biology Laboratory
The lab provides support for a range of soil microbial analyses, including analyses and training regarding mycorrhizal fungi and molecular ecology. The lab conducts Mycorrhizal Spore Count and Mycorrhizal Inoculum Potential (MIP) Bioassay to estimate the activities of mycorrhizal fungi in samples. The molecular ecology component of the core facility has worked with companies and academic labs to enumerate specific microorganisms in soils. This typically involves the extraction of DNA and enumeration of specific genes by quantitative PCR. More information about the Microbial Ecology and Molecular Biology Laboratory is here.
Training on mycorrhizal associations is organized by the laboratory every year during the summer on campus to help organic farmers, scientists, researchers, students, and others. Off-campus training sessions can also be arranged for an additional fee.
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Organic Contaminants Analytical Research Laboratory
The Organic Contaminants Analytical Research Laboratory (OCARL) provides services for faculty and students studying fate and transport of contaminants in soils and waters, including endocrine-disrupting compounds (EDCs), pharmaceuticals and personal care products, degradation metabolites of nonionic surfactants used in household and industrial detergents, petroleum refining (DNAPL and LNAPL), pulp and paper production, crop protection chemicals, waste products from plastics and textiles manufacturing. The OCARL is equipped with a high pressure liquid chromatograph with UV-Visible detector and a high pressure liquid chromatograph coupled to a triple quadrupole mass spectrometer (LC-MS/MS), two gas chromatographs with various detectors (nitrogen-phosphorus, electron capture, thermal conductive, and flame ionization), and a UV-visible spectrometer. The radioactive laboratory is used predominantly for radio-labeled contaminant degradation studies.
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Stable Isotope Mass Spectrometry Laboratory
Stable Isotope Mass Spectrometry Laboratory (SIMS)
The Stable Isotope Mass Spectrometry Laboratory (SIMS) is located in the Department of Soil, Water,
and Ecosystem Sciences (SWES) at the University of Florida. The SWS SIMS laboratory maintains and
operates a Thermo Delta V Advantage Isotope Ratio Mass Spectrometer (IRMS) interfaced via a
Conflo-IV device to a Costech ECS 4010 elemental analyzer for continuous flow measurement of stable
carbon (C) and nitrogen (N) isotope ratios of organic and inorganic samples. Additional peripherals
interfaced with the mass spec include a ThermoFinnigan GasBench II.
Mission:
The mission of the SWS Stable Isotopic Mass Spec Lab is to provide high quality stable isotopic
data to lab users at the University of Florida SWES Department and outside clients.
Coordinator: Kathryn Curtis
Scope of Services:
• The SWES Stable Isotope Mass Spec Lab can provide stable isotopic data (¹⁵N and ¹³C – both
natural abundance and enriched), as well as total nitrogen and total carbon data for soil, plant,
and other organic and inorganic materials using a Delta plus XL coupled to a Costech ECS 4010
elemental analyzer.
• The SWES Stable Isotope Mass Spec Lab can provide stable isotopic analysis of ¹³C of pure CO₂
or atmospheric CO₂ using a GasBench prep system coupled to the Delta plus XL.
• The SWES Stable Isotope Mass Spec Lab can provide stable isotopic analysis of ¹³C of dissolved
inorganic carbon (DIC) of water using a GasBench prep system coupled to the Delta plus XL
• The SWES Stable Isotope Mass Spec Lab also has available a microbalance for weighing and
loading samples in preparation for isotopic analysis. -
Wetland Biogeochemistry Laboratory
The Wetland Biogeochemistry Laboratory is a NELAP certified facility in the category of General Chemistry for water, soil, and plant tissue samples. Two laboratories provide instrumentation and equipment for a range of sample processing, digestion, and chemical analysis tasks. The sample processing and digestion lab is equipped with a muffle furnace and two acid resistant fume hoods for hot plate and tube/block digestions, as well as a fiber analyzer for plant tissue. The analytical lab houses three flow analyzers for nitrogen and phosphorus analysis, four gas chromatographs, an ion chromatograph (Dionex), a CN analyzer (Thermo Flash EA), a dissolved carbon analyzer, and other instruments such as UV/Vis and fluorescence spectrophotometers. The WBL also houses a Membrane Inlet Mass Spectrometry for measurement of denitrification rates in wetlands and aquatic systems. The laboratory also offers a variety of chemical and biochemical based analyses to characterize and quantify microbial community structure (Phospholipid Fatty Acid Analysis, PLFA), and microbial functions (fluoresce based enzyme analyses, denitrification assays, respiration (methane, CH4 and carbon dioxide, CO2). The gas chromatographs are configured to measure three different greenhouse gases nitrous oxide, methane and carbon dioxide.